Monday 18 June 2012

Biodiesel from Algae


Biodiesel Fuel
Biodiesel refers to a vegetable oil- or animal fat-based diesel fuel consisting of long-chain alkyl (methyl, propyl or ethyl) esters. Biodiesel is typically made by chemically reacting lipids (e.g., vegetable oil, animal fat (tallow)) with an alcohol producing fatty acid esters. Biodiesel is meant to be used in standard diesel engines and is thus distinct from the vegetable and waste oils used to fuel converted diesel engines. Biodiesel can be used alone, or blended with petro diesel. Biodiesel can also be used as a low carbon alternative to heating oil.
Biodiesel fuel is a clean burning alternative fuel that comes from 100% renewable resources. Many people believe that Biodiesel is the fuel of the future. Sometimes it is also known as Biofuel.  Biodiesel does not contain petroleum, but petroleum can be mixed to produce a biodiesel blend (eg. B20, B50) that can be used in many different vehicles. Pure biodiesel fuel (i.e. B100) though, can only be used in diesel engines.  Biodiesel is biodegradable and non-toxic, making it so safe that it is even safer than the commonly used table salt! Biodiesel is not like vegetable oil alternative fuels.  Biodiesel can be used in its unaltered form in diesel engines.  Vegetable oil fuels must be modified and used only in combustion-ignition engines.  This makes biodiesel one of the easiest alternative fuels to use.  In fact, it is a great option for use on farms in farm equipment. Biodiesel fuel is made through a process called Trans esterification.  This process involves removing the glycerin from the vegetable oil or fat.  During the process byproducts are left behind, including methyl esters and glycerin.  Biodiesel is free from such substances as sulfur and aromatics which are found in traditional fuels. Compared to other alternative fuels, biodiesel has a number of unique features and qualities. It has passed all the health effects testing requirements, unlike other alternative fuels.  This means it meets the standards of the 1990 Clean Air Act Amendments.  |The Environmental Protection Agency (EPA) has legally allowed Biodiesel to be sold and commercially distributed.  The rest of the alternative fuels cannot be sold commercially as motor fuel because they do not meet the EPA's fuel specifications. Biodiesel is also good for the economy because unlike traditional fuels, the resources to make biodiesel come from within the United States.  It is made with products grown in the USA without having to involve politics with other countries.  The country can become less dependent upon foreign countries for fuel supplies and the money goes right back into the US economy. Biodiesel Fuel Biodiesel an innovative fuel that is rapidly becoming more available to the general public. It can be found around the country in select places or it can be bought directly from producers. It costs a little more than traditional fuels at the current time because the demand is not as great.
Algae fuel
Algae fuel might be an alternative to fossil fuel and uses algae as its source of natural deposits. Several companies and government agencies are funding efforts to reduce capital and operating costs and make algae fuel production commercially viable. Harvested algae, like fossil fuel, release CO2 when burnt but unlike fossil fuel the CO2 is taken out of the atmosphere by the growing algae. High oil prices, competing demands between foods and other biofuel sources, and the world food crisis, have ignited interest in algaculture (farming algae) for making vegetable oil, biodiesel, bioethanol, bio gasoline, bioethanol, biobutanol and other biofuels, using land that is not suitable for agriculture. Among algal fuels' attractive characteristics: they do not affect fresh water resources, can be produced using ocean and wastewater, and are biodegradable and relatively harmless to the environment if spilled. Algae cost more per unit mass (as of 2010, food grade algae costs ~$5000/tonne), due to high capital and operating costs, yet are claimed to yield between 10 and 100 times more energy per unit area than other second-generation biofuel crops. One biofuels company has claimed that algae can produce more oil in an area the size of a two car garage than a football field of soybeans, because almost the entire algal organism can use sunlight to produce lipids, or oil.[8] The United States Department of Energy estimates that if algae fuel replaced all the petroleum fuel in the United States, it would require 15,000 square miles (39,000 km2) which is only 0.42% of the U.S. map, or about half of the land area of Maine. This is less than 1⁄7 the area of corn harvested in the United States in 2000.[10] However, these claims remain unrealized, commercially. According to the head of the Algal Biomass Organization algae fuel can reach price parity with oil in 2018 if granted production tax credits.
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Figure: Algae
Advantages of Biodiesel from Algae oil

·         Producing biodiesel from algae has been touted as the most efficient way to make biodiesel fuel. The main advantages of deriving biodiesel from algae oil include:
·         rapid growth rates,
·         a high per-acre yield (7 to 31 times greater than the next best crop – palm oil),
Algae have the potential to yield greater volumes of biofuel per acre of production than other biofuel sources. Algae could yield more than 2000 gallons of fuel per acre per year of production. Approximate yields for other fuel sources are far lower:
- Palm — 650 gallons per acre per year
- Sugar cane — 450 gallons per acre per year
- Corn — 250 gallons per acre per year
- Soy — 50 gallons per acre per year
·         certain species of algae can be harvested daily
·         algae biofuel contains no sulphur,
·         algae biofuel is non-toxic,
·         algae biofuel is highly bio-degradable, and
·         algae consume carbon dioxide as they grow, so they could be used to capture CO2 from power stations and other industrial plant that would otherwise go into the atmosphere.
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Figure: The Algae Bio factory
Factors
Dry mass factor is the percentage of dry biomass in relation to the fresh biomass; e.g. if the dry mass factor is 5%, one would need 20 kg of wet algae (algae in the media) to get 1 kg of dry algae cells.
Lipid content is the percentage of oil in relation to the dry biomass needed to get it, i.e. if the algae lipid content is 40%, one would need 2.5 kg of dry algae to get 1 kg of oil.
Fuels
The vegoil algae product can then be harvested and converted into biodiesel or green-colored crude oil. The algae’s carbohydrate content can be fermented into bioethanol and biobutanol.
Biodiesel
Currently most research into efficient algal-oil production is being done in the private sector, but predictions from small-scale production experiments bear out that using algae to produce biodiesel may be the only viable method by which to produce enough automotive fuel to replace current world diesel usage. If algae-derived biodiesel were to replace the annual global production of 1.1bn tons of conventional diesel then a land mass of 57.3 million hectares would be required, which would be highly favorable compared to other biofuels.  Microalgae have much faster growth rates than terrestrial crops. The per unit area yield of oil from algae is estimated to be from between 1,000 to 6,500 US gallons per acre per year (4,700 to 18,000 m3/km2·a). This is 7 to 30 times greater than the next best crop, Chinese tallow (700 US gal/area or 650 m3/km2·a). Studies show that some species of algae can produce up to 60% of their dry weight in the form of oil. Because the cells grow in aqueous suspension, where they have more efficient access to water, CO2 and dissolved nutrients, microalgae are capable of producing large amounts of biomass and usable oil in either high rate algal ponds or photo bioreactors. This oil can then be turned into biodiesel which could be sold for use in automobiles. Regional production of microalgae and processing into biofuels will provide economic benefits to rural communities.
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Figure: Algae Biodiesel Cycle

Biobutanol
Butanol can be made from algae or diatoms using only a solar powered bio refinery. This fuel has an energy density 10% less than gasoline, and greater than that of either ethanol or methanol. In most gasoline engines, butanol can be used in place of gasoline with no modifications. In several tests, butanol consumption is similar to that of gasoline, and when blended with gasoline, provides better performance and corrosion resistance than that of ethanol or E85.  The green waste left over from the algae oil extraction can be used to produce butanol.
Biogasoline
Bio gasoline is gasoline produced from biomass such as algae. Like traditionally produced gasoline, it contains between 6 (hexane) and 12 (dodecane) carbon atoms per molecule and can be used in internal-combustion engines.

Methane
Methane a form of natural gas can be produced from algae in various methods, namely Gasification, Pyrolysis and Anaerobic Digestion. In Gasification and Pyrolysis methods methane is extracted under high temperature and pressure. Anaerobic Digestion is a straight forward method involved in decomposition of algae into simple components then transforming it into fatty acids using microbes like acidific bacteria followed by removing any solid particles and finally adding methanogenic bacteria to release a gas mixture containing methane.
Ethanol
The Algenol system which is being commercialized by Bio Fields in Puerto Libertad, Sonora, Mexico utilizes seawater and industrial exhaust to produce ethanol.
SVO
The algal-oils feedstock that is used to produce biodiesels can also be used for fuel directly as "Straight Vegetable Oil", (SVO). The benefit of using the oil in this manner is that it doesn't require the additional energy needed for transesterification, (processing the oil with an alcohol and a catalyst to produce biodiesel). The drawback is that it does require modifications to a normal diesel engine. Transesterified biodiesel can be run in an unmodified modern diesel engine, provided the fuel system is using all non-rubber lines, O-rings and seals, which accounts for most diesel vehicles made after 1993. Viton is the best rubber-substitute for older diesel vehicle fuel lines and components when running biodiesel. As of 2006, the new standard for petroleum diesel in the United States is ultra-low sulfur diesel.

Hydrocracking to traditional transport fuels
Vegetable oil can be used as feedstock for an oil refinery where methods like hydrocracking or hydrogenation can be used to transform the vegetable oil into standard fuels like gasoline and diesel.

Jet fuel
Rising jet fuel prices are putting severe pressure on airline companies, creating an incentive for algal jet fuel research. The International Air Transport Association, for example, supports research, development and deployment of algal fuels. IATA’s goal is for its members to be using 10% alternative fuels by 2017.
Trials have been carried with aviation biofuel by Air New Zealand, Lufthansa, and Virgin Airlines.
In February 2010, the Defense Advanced Research Projects Agency announced that the U.S. military was about to begin large-scale production oil from algal ponds into jet fuel. After extraction at a cost of $2 per gallon, the oil will be refined at less than $3 a gallon. A larger-scale refining operation, producing 50 million gallons a year, is expected to go into production in 2013, with the possibility of lower per gallon costs so that algae-based fuel would be competitive with fossil fuels. The projects, run by the companies SAIC and General Atomics, are expected to produce 1,000 gallons of oil per acre per year from algal ponds.
Algae cultivation
Algae can produce up to 300 times more oil per acre than conventional crops, such as rapeseed, palms, soybeans, or jatropha. As algae have a harvesting cycle of 1–10 days, it permits several harvests in a very short time frame, a differing strategy to yearly crops (Chisti 2007). Algae can also be grown on land that is not suitable for other established crops, for instance, arid land, land with excessively saline soil, and drought-stricken land. This minimizes the issue of taking away pieces of land from the cultivation of food crops (Schenk et al. 2008). Algae can grow 20 to 30 times faster than food crops.
Photo bioreactors
Most companies pursuing algae as a source of biofuels are pumping nutrient-laden water through plastic or borosilicate glass tubes (called "bioreactors" ) that are exposed to sunlight (and so called photo bioreactors or PBR). Running a PBR is more difficult than an open pond, and more costly, but also more effective. Algae can also grow on marginal lands, such as in desert areas where the groundwater is saline, rather than utilize fresh water. Because algae strains with lower lipid content may grow as much as 30 times faster than those with high lipid content, the difficulties in efficient biodiesel production from algae lie in finding an algal strain, with a combination of high lipid content and fast growth rate, that isn't too difficult to harvest; and a cost-effective cultivation system (i.e., type of photo bioreactor) that is best suited to that strain. There is also a need to provide concentrated CO2 to increase the rate of production.
Closed loop system
Another obstacle preventing widespread mass production of algae for biofuel production has been the equipment and structures needed to begin growing algae in large quantities. Maximum use of existing agriculture processes and hardware is the goal. In a closed system (not exposed to open air) there is not the problem of contamination by other organisms blown in by the air. The problem for a closed system is finding a cheap source of sterile CO2. Several experimenters have found the CO2 from a smokestack works well for growing algae. To be economical, some experts think that algae farming for biofuels will have to be done as part of cogeneration, where it can make use of waste heat, and help soak up pollution.
Open pond
Open-pond systems for the most part have been given up for the cultivation of algae with high-oil content. Many believe that a major flaw of the Aquatic Species Program was the decision to focus their efforts exclusively on open-ponds; this makes the entire effort dependent upon the hardiness of the strain chosen, requiring it to be unnecessarily resilient in order to withstand wide swings in temperature and pH, and competition from invasive algae and bacteria. Open systems using a monoculture are also vulnerable to viral infection. The energy that a high-oil strain invests into the production of oil is energy that is not invested into the production of proteins or carbohydrates, usually resulting in the species being less hardy, or having a slower growth rate. Algal species with a lower oil content, not having to divert their energies away from growth, have an easier time in the harsher conditions of an open system. Research into algae for the mass-production of oil is mainly focused on microalgae; organisms capable of photosynthesis that are less than 0.4 mm in diameter, including the diatoms and cyanobacteria; as opposed to microalgae, such as seaweed. The preference towards microalgae is due largely to its less complex structure, fast growth rate, and high oil content (for some species). However, some research is being done into using seaweeds for biofuels, probably due to the high availability of this resource.
The following species listed are currently being studied for their suitability as a mass-oil producing crop, across various locations worldwide
The amount of oil each strain of algae produces is extremely different. To put it in perspective a list of microalgae and its’ various oil yields are listed below:
  • Ankistrodesmus TR-87: 28-40 % dw
  • Botryococcus braunii: 29-75 % dw
  • Chlorella sp.: 29%dw
  • Chlorella protothecoides(autotrophic/ heterothrophic): 15-55% dw
  • Cyclotella DI- 35: 42%dw
  • Dunaliella tertiolecta : 36-42%dw
  • Hantzschia DI-160: 66%dw
  • Nannochloris: 31(6-63)%dw
  • Nannochloropsis : 46(31-68)%dw
  • Nitzschia TR-114: 28-50%dw
  • Phaeodactylum tricornutum: 31%dw
  • Scenedesmus TR-84: 45%dw
  • Stichococcus: 33(9-59)%dw
  • Tetraselmis suecica: 15-32%dw
  • Thalassiosira pseudonana: (21-31)%dw
  • Crpthecodinium cohnii: 20%dw
  • Neochloris oleoabundans: 35-54%dw
  • Schiochytrium 50-77%dw

In addition, due to its high growth rate,
Ulva has been investigated as a fuel for use in the SOFT cycle, (SOFT stands for Solar Oxygen Fuel Turbine), a closed-cycle power generation system suitable for use in arid, subtropical regions.
Specific research
Companies such as Sapphire Energy and Bio Solar Cells are using genetic engineering to make algae fuel production more efficient. According to Klein Lankhorst of Bio Solar Cells, genetic engineering could vastly improve algae fuel efficiency as algae can be modified to only build short carbon chains instead of long chains of carbohydrates. Sapphire Energy also uses chemically induced mutations to produce algae suitable for use as a crop.
Some commercial interests into large-scale algal-cultivation systems are looking to tie in to existing infrastructures, such as cement factories,  coal power plants, or sewage treatment facilities. This approach changes wastes into resources to provide the raw materials, CO2 and nutrients, for the system.
Aquaflow Bionomic Corporation of New Zealand announced that it has produced its first sample of homegrown bio-diesel fuel with algae sourced from local sewerage ponds. A small quantity of laboratory produced oil was mixed with 95% regular diesel.
A feasibility study using marine microalgae in a photo bioreactor is being done by The International Research Consortium on Continental Margins at the Jacobs University Bremen.
Nutrients
Nutrients like nitrogen (N), phosphorus (P), and potassium (K), are important for plant growth and are essential parts of fertilizer. Silica and iron, as well as several trace elements, may also be considered important marine nutrients as the lack of one can limit the growth of, or productivity in, an area.


Carbon Dioxide
Bubbling CO2 through algal cultivation systems can greatly increase productivity and yield (up to a saturation point). Typically, about 1.8 tonnes of CO2 will be utilised per tonne of algal biomass (dry) produced, though this varies with algae species. The Glenturret Distillery in Perthshire, UK – home to The Famous Grouse Whisky – percolate CO2 made during the whisky distillation through a microalgae bioreactor. Each tonne of microalgae absorbs two tonnes of CO2. Scottish Bioenergy, who run the project, sell the microalgae as high value, protein-rich food for fisheries. In the future, they will use the algae residues to produce renewable energy through anaerobic digestion.
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Figure: Bioreactor System


Wastewater
A possible nutrient source is waste water from the treatment of sewage, agricultural, or flood plain run-off, all currently major pollutants and health risks. However, this waste water cannot feed algae directly and must first be processed by bacteria, through anaerobic digestion. If waste water is not processed before it reaches the algae, it will contaminate the algae in the reactor, and at the very least, kill much of the desired algae strain. In biogas facilities, organic waste is often converted to a mixture of carbon dioxide, methane, and organic fertilizer. Organic fertilizer that comes out of the digester is liquid, and nearly suitable for algae growth, but it must first be cleaned and sterilized.
The utilization of wastewater and ocean water instead of freshwater is strongly advocated due to the continuing depletion of freshwater resources. However, heavy metals, trace metals, and other contaminants in wastewater can decrease the ability of cells to produce lipids biosynthetically and also impact various other workings in the machinery of cells. The same is true for ocean water, but the contaminants are found in different concentrations. Thus, agricultural-grade fertilizer is the preferred source of nutrients, but heavy metals are again a problem, especially for strains of algae that are susceptible to these metals. In open pond systems the use of strains of algae that can deal with high concentrations of heavy metals could prevent other organisms from infesting these systems (Schenk et al. 2008). In some instances it has even been shown that strains of algae can remove over 90% of nickel and zinc from industrial wastewater in relatively short periods of time (Chong, Wong et al. 1998).
Investment and economic viability
There is always uncertainty about the success of new products and investors have to consider carefully the proper energy sources in which to invest.  A drop in fossil fuel oil prices might make consumers and therefore investors lose interest in renewable energy. Algal fuel companies are learning that investors have different expectations about returns and length of investments. AlgaePro Systems found in its talks with investors that while one wants at least 5 times the returns on their investment, others would only be willing to invest in a profitable operation over the long term. Every investor has its own unique stipulations that are obstacles to further algae fuel development. Additional concerns consider the potential environmental impact of Algal fuel development, as well as secondary impacts on wildlife such as bears and fish.
Whereas technical problems, such as harvesting, are being addressed successfully by the industry, the high up-front investment of algae-to-biofuels facilities is seen by many as a major obstacle to the success of this technology. Only few studies on the economic viability are publicly available, and must often rely on the little data (often only engineering estimates) available in the public domain. Dmitrov examined the Green Fuels photo bioreactor and estimated that algae oil would only be competitive at an oil price of $800 per barrel. A study by Alabi at al. examined raceways, photo bioreactors and anaerobic fermenters to make biofuels from algae and found that photo bioreactors are too expensive to make biofuels. Raceways might be cost-effective in warm climates with very low labor costs, and fermenters may become cost-effective subsequent to significant process improvements. The group found that capital cost, labor cost and operational costs (fertilizer, electricity, etc.) by themselves are too high for algae biofuels to be cost-competitive with conventional fuels. Similar results were found by others, suggesting that unless new, cheaper ways of harnessing algae for biofuels production are found, their great technical potential may never become economically accessible. Recently, Rodrigo E. Teixeira demonstrated a new reaction and proposed a process for harvesting and extracting raw materials for biofuel and chemical production that requires a fraction of the energy of current methods, while extracting all cell constituents.
Even with these difficulties some companies have managed to mass produce algae. Highlighted below are Blue Marble and Solazyme. These two companies have made significant progress in either environmentally safe practices or producing enough algae for the mass production of biofuel.
Engineering design and cost studies have been done throughout the course of the ASP, with ever increasing realism in the design assumptions and cost estimates. The last set of cost estimates for the program was developed in 1995. These estimates showed that algal biodiesel cost would range from $1.40 to $4.40 per gallon based on current and long-term projections for the performance of the technology. Even with assumptions of $50 per ton of CO2 as a carbon credit, the cost of biodiesel never competes with the projected cost of petroleum diesel.
Description: COM
Figure: $ per Barrel

Blue Marble Production
Blue Marble Production is a Seattle based company that is dedicated to removing alga from algae-infested water. This in turn cleans up the environment and allows this company to produce biofuel. Rather than just focusing on the mass production of algae, this company focuses on what to do with the byproducts. This company recycles almost 100% of its water via reverse osmosis, saving about 26,000 gallons of water every month . This water is then pumped back into their system. The gas produced as a byproduct of algae will also be recycled by being placed into a photo bioreactor system that holds multiple strains of algae. Whatever gas remains is then made into pyrolysis oil by thermochemical processes. Not only does this company seek to produce biofuel, but it also wishes to use algae for a variety of other purposes such as fertilizer, food flavoring, anti-inflammatory, and anti-cancer drugs.
Solazyme
Solazyme is one of a hand full of companies which is supported by oil companies such as Chevron. Additionally, this company is also backed by Imperium Renewables, Blue Crest Capital Finance, and The Roda Group. Solazyme has developed a way to use up to 75% percent of dry algae as oil .This process requires the algae to grow in a dark fermentation vessel and be fed by carbon substrates within their growth media. The effect is the production of triglycerides that are almost identical to vegetable oil. Solazyme’s production method is said to produce more oil than those algae cultivated via photosynthetically or made to produce ethanol. Oil refineries can then take this algal oil and turn it into biodiesel, renewable diesel or jet fuels. Part of Solazyme’s Maersk Line was a test, in collaboration with the U.S Navy, which placed 100% algae fuel into 300- meter Maersk container vessel . This 100% algae fuel was used in a test to sail from Northern Europe to Indonesia. This voyage was highly successful. Using 300 tons of algae based fuel, these carriers made the one month voyage. The next test would be a 50/50, gasoline and algae biofuel, mix for a “Green Strike Group” that should be in operation by summer 2012.
Algae fuel by country
Europe
Universities in the United Kingdom which are working on producing oil from algae include:University of Glasgow, University of Brighton, Cambridge University, University College London, Imperial College London, Cranfield University and Newcastle University. In Spain, it is also relevant the research carried out by the CSIC´s Instituto de Bioquímica Vegetal y Fotosíntesis (Microalgae Biotechnology Group, Seville).
Ukraine plans to produce biofuel using a special type of algae.
United States
The Aquatic Species Program, launched in 1978, was a research program funded by the United States Department of Energy (DoE) which was tasked with investigating the use of algae for the production of energy. The program initially focused efforts on the production of hydrogen, shifting primary research to studying oil production in 1982. From 1982 until its end in 1996, the majority of the program research was focused on the production of transportation fuels, notably biodiesel, from algae. In 1995, as part of overall efforts to lower budget demands, the DoE decided to end the program. Research stopped in 1996 and staff began compiling their research for publication.
At the Woods Hole Oceanographic Institution and the Harbor Branch Oceanographic Institution the wastewater from domestic and industrial sources contain rich organic compounds that are being used to accelerate the growth of algae. The Department of Biological and Agricultural Engineering at University of Georgia is exploring microalgal biomass production using industrial wastewater.  Algaewheel, based in Indianapolis, Indiana, presented a proposal to build a facility in Cedar Lake, Indiana that uses algae to treat municipal wastewater, using the sludge byproduct to produce biofuel.
Sapphire Energy (San Diego) has produced green crude from algae.
Solazyme (South San Francisco, California) has produced a fuel suitable for powering jet aircraft from algae.
Other
Global air carriers Air New Zealand, Continental, Virgin Atlantic Airways, and biofuel technology developer UOP, a Honeywell company, will be the first wave of aviation-related members, together with Boeing, to join Algal Biomass Organization.
The National Algae Association (NAA) is a non-profit organization of algae researchers, algae production companies and the investment community who share the goal of commercializing algae oil as an alternative feedstock for the biofuels markets. The NAA gives its members a forum to efficiently evaluate various algae technologies for potential early stage company opportunities.
The European Algae Biomass Association (EABA) is the European association representing both research and industry in the field of algae technologies, currently with 79 members. The association is headquartered in Florence, Italy. The general objective of the European Algae Biomass Association (EABA) is to promote mutual interchange and cooperation in the field of biomass production and use, including biofuels uses and all other utilisations. It aims at creating, developing and maintaining solidarity and links between its Members and at defending their interests at European and international level. Its main target is to act as a catalyst for fostering synergies among scientists, industrialists and decision makers in order to promote the development of research, technology and industrial capacities in the field of Algae.
Pond Biofuels Inc. in Canada has grown algae directly off of a cement plant smokestack emission, and used waste heat to dry the algae, as well.
Ocean Nutrition Canada in Halifax, Nova Scotia, Canada has found a new strain of algae that appears capable of producing oil at a rate 60 times greater than other types of algae being used for the generation of biofuels.
VG Energy, a subsidiary of Viral Genetics Incorporated, claims to have discovered a new method of increasing algal lipid production by disrupting the metabolic pathways that would otherwise divert photosynthetic energy towards carbohydrate production. Using these techniques, the company states that lipid production could be increased several-fold, potentially making algal biofuels cost-competitive with existing fossil fuels.
Algae production from the warm water discharge of a nuclear power plant has been piloted by Patrick C. Kangas at Peach Bottom Atomic Power Station, owned by Exelon Corporation. This process takes advantage of the relatively high temperature water to sustain algae growth even during winter months.



Reference:
·         en.wikipedia.org/wiki/Algae_fuel

   Cornell, Clayton B. (2008-03-29). "First Algae Biodiesel Plant Goes Online: April 1, 2008". Gas 2.0. Retrieved 2008-06-10.
  "'Green Dream' Backed by MPs". Eastern Daily Press. January 2003. Retrieved 2008-06-10.
  "Low Cost Algae Production System Introduced". Energy-Arizona. 2007-08-28. Retrieved 2008-06-10.
  Greenwell et al (2010) Placing microalgae on the biofuels priority list: a review of the technological challenges J. R. Soc. Interface 6 May 2010 vol. 7 no. 46 703-726
  "Why Algae?". Solix Biofuels. Archived from the original on June 9, 2008. Retrieved 2008-06-11.
   Hartman, Eviana (2008-01-06). "A Promising Oil Alternative: Algae Energy". The Washington Post. Retrieved 2008-06-10.
   Dyer, Gwynne (2008-06-17). "A replacement for oil". The Chatham Daily News. Retrieved 2008-06-18.
·         www.cifor.org/biofuel
·         www.oilgae.com/

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